Methanococcus jannaschii sRNA Annotation

Name PE Conf Guide Box Target RNA Target Position Match/Mismatch Notes
sR1  D' 23S G2008 11/0  
    D' 23S G2537 9/0  
    D 23S C2582 11/0 Non-CDP 
sR2  D' 23S U2529 9/1  
    D 23S C2649 13/0 CDP 
sR3  D' 23S C2020 10/0 CDP 
    D 23S G2049 10/0 CDP 
sR4  D' 23S G1796 10/0  
    D' 23S G1341 10/0  
    D 23S C2094 10/0 Non-CPD 
sR5  D 16S C1209 11/0  
    D 23S C583 10/0  
sR6  D' 23S C2034 12/0 CDP 
    D 23S G1318 11/1  
sR7  D' 23S U2591 11/0  
    D 23S G50 11/0 CDP 
sR8  D' 23S G900 11/0  
    D' tRNA Met C34 10/0  

PE Conf: the presence of the sRNA in total cellular RNA was verified by primer extension. Guide Box: the position of the guide within the sRNA (D or D' box associated). Target RNA: predicted target of methylation. Target Position: predicted nucleotide postion of methylation. Match/Mismatch: the number of Watson-Crick and G-U matches / mismatches within the sRNA-target RNA duplex. Notes: "CDP", dNTP concentration-dependent primer extension pause observed at the predicted site of methylation, indicating a likely 2'-O-ribose methyl; "No Pause", no pause was detected at either high or low dNTP concentrations. Guides without notation were not experimentally examined.